Short communication: Quantification of carbohydrates in whey permeate products using high-performance anion-exchange chromatography with pulsed amperometric detection

Analysis of Carbohydrates by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)

Anion-Exchange Chromatography I. Mechanism of Separation Although anion-exchange chromatography has been used extensively to analyze acidic carbohydrates and glycopeptides, it has not been commonly used for analysis of neutral sugars. However, examination of the pKa values of the neutral monosaccharides listed in Table 1 shows that carbohydrates are in fact weak acids. At high pH, they are at l...

Rapid Determination of Lactose and Lactulose in Dairy Products Using High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

Determination of mono- and disaccharides in milk and milk products by high-performance anion-exchange chromatography with pulsed amperometric detection

A simple and sensitive liquid chromatographic method for the separation and quantification of monoand disaccharides in rawand processed-milk is described. Samples of cows’, buffalos’, sheeps’ and goats’ milk were analyzed upon clarification and appropriate dilution for the quantification of lactose, galactose, glucose and N-acetylglucosamine (GlcNAc). The separation was accomplished by high-per...

Determination of Sucralose in Reduced- Carbohydrate Colas using High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

INTRODUCTION Sucralose (trichlorogalactosucrose or 1,6-dichloro1,6-dideoxy-b-D-fructofuranosyl-4-deoxy-a-D-galactopyranoside) is a non-nutritive sweetener used to manufacture diabetic and dietetic foods and beverages. Detection of sucralose and other carbohydrates is challenging because they lack a strong chromophore and, therefore, cannot be detected at low concentrations with UV detection. Fu...

Identification of tobramycin impurities for quality control process monitoring using high-performance anion-exchange chromatography with integrated pulsed amperometric detection.

Commercial-scale fermentation for tobramycin manufacture is carried out with Streptomyces tenebrarius. Impurity profiling during various phases of pharmaceutical production is important for evaluating the effectiveness of a processing step and meeting regulatory requirements. High-performance anion-exchange (HPAE) chromatography with integrated pulsed amperometric detection (HPAE-IPAD) is a hig...